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Addgene inc
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Genechem
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Image Search Results
Journal: Neuron
Article Title: Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells
doi: 10.1016/j.neuron.2019.05.044
Figure Lengend Snippet: (A and B) Images of optic nerve sections showing CTB-labeled axons (grey) in C57BL/6J mice injected with either (A) AAV-THBS1 or (B) AAV-GFP. Asterisks, lesion site. Scale bars, 100 μm.
Article Snippet: To generate the pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA) plasmid, the 377 bp CMV promoter coding sequence was deleted from pAAV.CMV.SV40.THBS1-HA.SV40(
Techniques: Labeling, Injection
Journal: Neuron
Article Title: Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells
doi: 10.1016/j.neuron.2019.05.044
Figure Lengend Snippet: (A) A schematic of THBS1 mutants investigated. All constructs contain the N-terminal signal peptide and have a C-terminal HA tag. Laminin G domain (LamG), oligomerization coiled coil (CC) domain, von Willebrand complex like domain (vWC), thrombospondin type 1 repeat domain (TSR1), epidermal growth factor-like repeat domains (EGF), type 3 repeat domain (TSR3), and the thrombospondin C-terminal domain (CTD). THBS4 is shown for comparison to THBS1.
Article Snippet: To generate the pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA) plasmid, the 377 bp CMV promoter coding sequence was deleted from pAAV.CMV.SV40.THBS1-HA.SV40(
Techniques: Construct
Journal: Neuron
Article Title: Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells
doi: 10.1016/j.neuron.2019.05.044
Figure Lengend Snippet: (A) Images of optic nerve section showing GFP-labeled axons (green) from HB9:GFP;Bax−/− mice and CTB (magenta) following injection with AAV-THBS1 and optic nerve crush. Asterisks, lesion site.
Article Snippet: To generate the pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA) plasmid, the 377 bp CMV promoter coding sequence was deleted from pAAV.CMV.SV40.THBS1-HA.SV40(
Techniques: Labeling, Injection
Journal: Neuron
Article Title: Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells
doi: 10.1016/j.neuron.2019.05.044
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: To generate the pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA) plasmid, the 377 bp CMV promoter coding sequence was deleted from pAAV.CMV.SV40.THBS1-HA.SV40(
Techniques: shRNA, Recombinant, Multiplex Assay, Clone Assay, Software